Abstract: Purposes To evaluate the effects of HiLo training on pyruvic ketolase and the expression of HIF-1α and ChREBP in rat skeletal muscles. Methods After adaptive training, 40 8-week-old male SD rats are divided into living-low quiet control group(LC), living-low training-low group(LoLo), living-high quiet control group(HC) and living-high training-low group(HiLo). All living-high groups stay in the environment with 13.6% oxygen concentration, about the altitude of 3500 m, for 12h/day. All training groups undergo treadmill training with 35m/min for 1hour/day, 5days/ week, 4 weeks. Gastrocnemius is obtained after 24hs of last training. The activity of PK is detected by colorimetry. Technology of Real Time Quantitative PCR and Western blotting are adopted in order to test the genes expression and protein level changes of PK, HIF-1α and ChREBP. Results (1) Hypoxia reduces PK activity significantly (p<0.01). Training increases PK protein level significantly (p<0.05). Hypoxia and training increase PK mRNA level significantly (p<0.01) and there is significantly interaction. (2) Hypoxia and training increase HIF-1α mRNA and protein level significantly (p<0.01) and there is significant interaction. (3) Training increases ChREBP mRNA and protein level significantly (p<0.05 or p<0.01), and there is significantly interaction between hypoxia and training (p<0.01). Conclusions 4 weeks of HiLo training can reduce resting rat’s gastrocnemius activity of PK, and the up-regulation of gastrocnemius HIF-1α and ChREBP gene and protein expression can induce PK gene expression, which is beneficial to PK for the body to store energy in resting state and stimulating activity at state of motion, which is the result of HiLo training accommodation.

Key words: HiLo, PK, HIF-1α, ChREBP