Abstract: Objective: To study the effects of L-carnitine combined with exercise on FAT/CD36 and CPT-1 of obese mice. Methods: Male C57BL / 6 mice were randomly assigned to group C (fed with normal diet) and group H (fed with high-fat diet). After group H was modeled successfully, they were randomly divided into group H (obesity quiet), group HL (obesity with L-carnitine), group HE (obesity with exercise), group HLE (obesity with L-carnitine combined exercise). Swimming training was adopted to them, 1 hour / day, 5 days / week, lasting for five weeks. Group HL, HLE were gavaged with L-carnitine an hour before training. During the experiment, changes of body weight of mice were monitored. After the experiment, the gastrocnemius muscles were prepared to determine the content of FFA, CPT-1 and FAT/CD36. Results: ① After 5 weeks of training, the body weight of group HE and HLE became lower than group H, and the weight of group HLE was also lower than group HL; ② the FFA of group HL, HE and HLE was significantly lower than in group H, and in group HE and HLE it was also significantly lower than group HL; ③ the contents of CPT-1 of group HL, HE and HLE were significantly lower than in group H; ④ the expression of FAT/CD36 of group HE was higher than in group H, HLE group was lower than group H; ⑤ the content of CPT-1 and FAT/CD36 showed a moderate positive correlation with body weight. These differences were statistically significant, P <0.05 or P <0.01. Conclusion: ① there is no apparent role of pure L-carnitine supplementation in controlling weight. However, there is significant effect of exercise and exercise combined with L-carnitine on controlling weight. ② the effect of exercise and exercise combined with L-carnitine in promoting metabolism and oxidation of fatty acid is more obvious than pure L-carnitine supplementation. ③ L-carnitine, exercise, and exercise combined with L-carnitine can reduce CPT-1 levels. ④ exercise combined with L-carnitine can interactively reduce the expression of FAT/CD36. The contents of CPT-1, FAT/CD36 are moderately correlated with body weight.

Key words: L-carnitine, exercise, CPT-1, FAT/CD36