Abstract: Objective: To investigate the antagonism of high-frequency sine-wave vibration (HFV) on the apoptosis of skeletal muscle in the disused state. Methods: The animal models were established and the key techniques of the vibration instrument were improved. The short-term intermittent vibration was used to observe the effects of HFV on soleus muscle (SOL) wet weight in the brains of SD rats. The ultrasonography, immunohistochemical staining TUNEL assay, the effect of HFV on the brake in rat soleus muscle (SOL) wet weight to body weight ratio, muscle fiber cross-sectional area, ultrastructural changes, Bcl-2 and Bax expression. Results 1) The body weight ratio of SOL was 30.22 ± 2.68, decreased by 36.43%. The cross-sectional area of muscle fiber was 3789.56 ± 95.14μm², which decreased by 37.77%. (P<0.05). (2) Electron microscopy showed that the extraocular muscle lesion was obvious after the brachytherapy, and chromatin agglutination in the nucleus was observed in the HFV group compared with the brachytherapy group. Skeletal muscle part of the dissolution of the sarcolemma, nuclear deformation. (P<0.05), the Z-line structure of the extra-oculi muscle in the vibration group was clear, and no obvious change was observed in the intrafusal muscle fiber structure.3) The expression of Bcl-2 in the SOL was significantly lower than that in the control group <0.05). The positive rate of Bax expression was 80% after brakes, which was significantly higher than that of the control group (P<0.05). AI after braking was 20%, significantly higher than the control group (P<0.05). The expression of Bcl-2 and the expression of Bax and AI in the HFV group were significantly lower than those in the control group (P<0.05). Conclusion The modified dystrophy vibrator has strong operability, which can restrain the process of apoptosis of the brake muscular atrophy.

Key words: high frequency sinusoidal vibration, muscle spindle, disuse atrophy, apoptosis, skeletal muscle