Abstract: Objective: The purpose of this experiment is to investigate the effect of treadmill training of different intensity on the PPARγ/adiponectin/TNF-α mRNA in adipose tissue. Methods: Forty male SD rats are randomly assigned to four high fat diet groups. Rats in exercise groups are trained through treadmill exercise by SE,ME,HE. Rats in exercise groups undergo treadmill exercise for 10 weeks(60min/day,6days/week). At the end of experiment, all rats are killed and their blood, epididymal fat as well as perirenal fat are obtained. Atherosclerotic was observed. The PPARγ/adiponectin/TNF-α mRNA in adipose tissue are determined. Serum is tested by enzyme-linked immunosorbentassay. Results :The weight increase of rats in exercise groups is lower than that in group C, and the highest intensity has the most obvious effect(P<0.01. The serum TG,TC in group C are the highest in the four groups (P<0.01). Compared with groups ME HE, the serum TG,TC in group SE are lower (P<0.05). The serum HDL-C in group ME is the highest in the four groups (P<0.05). The level of PPARγ adiponectin mRNA expression in adipose tissue and the serum adiponectin of group ME are the highest, and group C is the lowest. No significant difference is found between group SE and group HE. The level of TNF-α mRNA expression in adipose tissue and the serum TNF-α of group C are the highest. Group ME is the lowest with no significant difference found between group SE and group HE. Conclusion: Loaded treadmill exercise with proper intensity has good effect on PPARγ,adiponectin mRNA expression in adipose tissue, which lowers the TNF-α mRNA expression in adipose tissue of rats fed with high fat diet. This indicates that exercise playes an active role in preventing excessive accumulation of fat induced by high fat diet.

Key words: exercise, adipose tissue, PPARγ, adiponectin, TNF-α