主办单位：成都体育学院
ISSN 1001-9154　CN 51-1097/G8
Journal of Chengdu Sport University

Journal of Chengdu Sport University ›› 2018, Vol. 44 ›› Issue (4): 121-126.doi: 10.15942/j.jcsu.2018.04.020

• PHYSICAL EDUCATION AND TRAINING· SPORTS SCIENCE OF HUMAN BODY • Previous Articles    

Effects of Different Intensity Exercise on AMPK/PGC-1α Signaling Pathway in Skeletal Muscle of Rats

XU Jie1, HUANG Qiaoting1, XIE Minhao2, YAN Yi3, LIN Jiashi4   

  1. 1.Chengdu University, Chengdu Sichuan 610106;
    2.Institute of Medicine of General Administration of Sport of China, Beijin 100061;
    3.Beijing Sport University, Beijing 100084;4.Jimei University, Xiamen Fujian 361021
  • Received:2017-12-17 Revised:2018-04-15 Online:2018-07-15 Published:2018-07-20

PDF (PC)

1006

Abstract: Objective: To investigate the biological mechanism of exercise to improve cardiorespiratory fitness (CRF) through exercise based on 12 weeks of exercise intervention with different intensities on the skeletal muscle AMPK/PGC-1α pathway in rats. Methods: 40 male Wistar rats were randomly divided into four groups with 10 in each group, i.e. sedentary group, low intensity group (50%O2max), moderate intensity group (65%O2max), and high intensity group (80% O2max). All rats were measured for the O2max after three days adaptive training. After that, the rats in the exercise groups were trained for 12 weeks, 5 day a week, and 1hour per day. They were tested for O2max every two weeks during the training period. All rats were sacrificed 48 hours after the last exercise and their soleus (right) was isolated. The AMPK and PGC-1αgene expression were detected by RT-qPCR while the AMPK,PGC-1α,and phosphorylated AMPK protein expression by Western Blot. Results: The O2max in 65% O2max group and 80% O2max group increased rapidly, while the O2max in 50%O2max group increased significantly in the late stage of intervention. After 12 weeks of intervention, the PGC-1α mRNA expression in 50%O2max group and 80%O2max group were significantly higher than that in sedentary group and moderate intensity group (P<0.01), the AMPK mRNA expression in sedentary group and 80%O2max group were significantly lower than that in 65%O2max group (P<0.05, P<0.01). The PGC-1α protein expression in the exercise groups was higher than that in sedentary group, with only the PGC-1α protein expression in 65%O2max group being significantly higher than that in sedentary group (P<0.05). There was no significant difference in AMPK protein expression among the groups, while the phosphorylated AMPK protein expression in moderate intensity group and high intensity group increased as compared with sedentary group. The increase in 80%O2max group was greater, significantly higher than that in sedentary group and low intensity group (P<0.05). Conclusions: Higher exercise intensity does not mean that PGC-1α protein expression will increase correspondingly. The effect of moderate intensity exercise on the AMPK/PGC-1α signaling pathway may be more beneficial for CRF.

Key words: AMPK, PGC-1α, exercise intensity, CRF

CLC Number: