低氧运动对胰岛素抵抗大鼠脂肪组织缺氧状况的影响

doi:10.15942/j.jcsu.2019.03.011

成都体育学院学报 ›› 2019, Vol. 45 ›› Issue (3): 69-74.doi: 10.15942/j.jcsu.2019.03.011

低氧运动对胰岛素抵抗大鼠脂肪组织缺氧状况的影响

陈圣菊¹, 尚画雨², 白胜超¹, 张荷¹, 张俊芬¹, 邱平学¹, 周越¹

1.北京体育大学运动人体科学学院,北京 100084;
2.成都体育学院运动医学与健康学院,四川成都 610041

收稿日期:2018-02-05 出版日期:2019-05-15 发布日期:2019-05-23
通讯作者: 周越,教授,博士,博士生导师,研究方向:运动生理学,E-mail: chowyue@163.com
作者简介:陈圣菊,在读博士,研究方向:运动对骨骼肌形态机能的影响,E-mail: chenshengju@yeah.net
基金资助:
中央高校基本科研业务费专项资金资助“离心运动对2型糖尿病大鼠肌卫星细胞增殖分化的影响”(2016ZD003)

Effects of Hypoxic Exercise on the Hypoxia of Adipose Tissue in Rats with Insulin Resistance

CHEN Shengju¹, SHANG Huayu², BAI Shengchao¹, ZHANG He¹, ZHANG Junfen¹, QIU Pingxue¹, ZHOU Yue¹

1.Beijing Sport University, Beijing 100084;
2. Chengdu Sport University, Chengdu Sichuan 610041

Received:2018-02-05 Online:2019-05-15 Published:2019-05-23

摘要/Abstract

摘要： 目的: 探讨缺氧对胰岛素抵抗大鼠脂肪组织的影响,为运动治疗肥胖和胰岛素抵抗提供新的理论依据。方法: 通过8周的高脂饮食筛选出符合建模标准的大鼠,建立胰岛素抵抗大鼠模型。将24只成模大鼠随机分为4组,每组6只:常氧安静组(NC)、常氧运动组(NE)、低氧安静组(HC)和低氧运动组(HE)。其中,NE和HE组大鼠采用水平动物跑台进行运动训练,HC和HE组大鼠施以13.7%氧浓度干预,实验期间隔天称量大鼠体重。实验结束后处死大鼠并剥离其肾周及睾周脂肪,称量重量。取睾周脂肪制作冰冻切片,行HE染色观察脂肪细胞直径的变化;Western Blot检测脂肪组织中HIF-1α和Glut-1的蛋白表达。结果: 4周低氧运动能够显著降低大鼠体重及增长幅度(P＜0.05)。NE和HE组大鼠肾周及睾周脂肪总含量显著低于其他组(P＜0.05),两组间无明显差异;各组大鼠的脂肪组织直径均显著低于对照组(P＜0.05)。运动及低氧能够显著降低脂肪组织中HIF-1α的蛋白表达(P＜0.05),对Glut-1的蛋白表达无明显影响。结论: 从减缓体重增加幅度及脂肪含量角度来看,低氧运动和常氧运动减体重的效果优于单纯低氧暴露。低氧和运动都能显著改善脂肪组织的缺氧状况,但对糖代谢无明显影响。常氧运动更有利于改善胰岛素抵抗个体中脂肪组织的缺氧状况。

关键词: 低氧运动, 胰岛素抵抗, 脂肪组织, 缺氧

Abstract: Objective: To explore the changes of a series of indexes of adipose tissue in hypoxic exercise in a high-fat diet (HFD) induced insulin resistance rats model, and preliminarily investigate the effects of hypoxia on adipose tissue in rats with insulin resistance, so as to provide a new theoretical basis for exercise treatment of obesity and insulin resistance. Methods: The rats were screened by a HFD for 8 weeks and a model of insulin resistance was established. The 24 rats were randomly divided into 4 groups: control group (NC, n=6), normoxic exercise group (NE), hypoxic quiet group (HC, n=6) and hypoxic exercise group (HE, n=6). The rats in NE and HE were trained by the horizontal animal running platform, and the rats in HC and HE were treated with 13.7% oxygen concentration, and the rats were weighted in the next day during the test period. After the experiment, the rats were killed. Their perirenal and epididymal adipose tissue were stripped and weighted. The changes of adipocyte diameter were observed by HE staining. Western blot detected the protein expression of HIF-1α and Glut-1 in adipose tissue. Results: 4-week hypoxic and exercise can significantly reduce the weight and increase of rats (P＜0.05). The total fat content of the rats in exercise groups was significantly lower than that of other groups (P＜0.05), while showed no significant difference between the two exercise groups (P＞0.05). The adipocyte diameter in each group was significantly lower than that in the control group (P＜0.05). Exercise and hypoxia can significantly reduce the expression of HIF-1α in adipose tissue (P＜0.05), which has no significant effect on the protein expression of Glut-1 (P＞0.05). Conclusion: From the perspective of slowing down the body weight gain and fat content, the effect of weight loss in normoxic exercise and hypoxic exercise are better than that of pure hypoxic exposure. Both hypoxia and exercise can significantly improve the status of anoxia in adipose tissue, but have no obvious effect on glucose metabolism. Normoxic exercise is more beneficial to improve the anoxia of adipose tissue in individuals with insulin resistance.

Key words: hypoxic exercise, insulin resistance, anoxia, adipose tissue

中图分类号:

G804.2

陈圣菊, 尚画雨, 白胜超, 张荷, 张俊芬, 邱平学, 周越. 低氧运动对胰岛素抵抗大鼠脂肪组织缺氧状况的影响[J]. 成都体育学院学报, 2019, 45(3): 69-74.

CHEN Shengju, SHANG Huayu, BAI Shengchao, ZHANG He, ZHANG Junfen, QIU Pingxue, ZHOU Yue. Effects of Hypoxic Exercise on the Hypoxia of Adipose Tissue in Rats with Insulin Resistance[J]. Journal of Chengdu Sport University, 2019, 45(3): 69-74.

[1]	李恩, 叶魁, 孙君志, 苏全生. 8周游泳运动对高脂饮食诱导胰岛素抵抗大鼠骨骼肌细胞自噬的影响[J]. 成都体育学院学报, 2019, 45(4): 121-126.
[2]	夏书宇. 不同强度跑台运动对高脂饮食大鼠脂肪组织PPARγ/脂联素/TNF-αmRNA的影响[J]. 成都体育学院学报, 2015, 41(3): 98-102.

低氧运动对胰岛素抵抗大鼠脂肪组织缺氧状况的影响

Effects of Hypoxic Exercise on the Hypoxia of Adipose Tissue in Rats with Insulin Resistance

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