不同强度运动对大鼠骨骼肌AMPK/PGC-1α信号通路的影响

doi:10.15942/j.jcsu.2018.04.020

成都体育学院学报 ›› 2018, Vol. 44 ›› Issue (4): 121-126.doi: 10.15942/j.jcsu.2018.04.020

• 体育教育训练学·运动人体科学 • 上一篇

不同强度运动对大鼠骨骼肌AMPK/PGC-1α信号通路的影响

许杰¹,黄巧婷¹,谢敏豪²,严翊³^*,林家仕⁴

1.成都大学,四川成都 610106;
2.国家体育总局运动医学研究所,北京100061;
3.北京体育大学,北京 100084;
4.集美大学,福建厦门 361021

收稿日期:2017-12-17 修回日期:2018-04-15 出版日期:2018-07-15 发布日期:2018-07-20
通讯作者: 严翊,博士,教授,研究方向:运动生物化学,E-mail:yanyi22@sina.com。
作者简介:许杰,博士,讲师,研究方向:运动与体质健康,E-mail:roby_xiao@sina.com。
基金资助:
国家“十二五”科技支撑计划课题“制定有效运动负荷方法与评价等级的研究”(2012BAK21B02);国家自然科学基金课题“PGC-1α信号级联在运动强度改善心肺耐力中的剂量-效应特征及调控机制研究”(31401017);教育部优秀博士论文课题(V200801)

Effects of Different Intensity Exercise on AMPK/PGC-1α Signaling Pathway in Skeletal Muscle of Rats

XU Jie¹, HUANG Qiaoting¹, XIE Minhao², YAN Yi³, LIN Jiashi⁴

1.Chengdu University, Chengdu Sichuan 610106;
2.Institute of Medicine of General Administration of Sport of China, Beijin 100061;
3.Beijing Sport University, Beijing 100084;4.Jimei University, Xiamen Fujian 361021

Received:2017-12-17 Revised:2018-04-15 Online:2018-07-15 Published:2018-07-20

摘要/Abstract

摘要： 目的: 通过12周不同强度运动干预,从大鼠骨骼肌AMPK/PGC-1α通路角度,研究运动改善心肺耐力的生物学机制。方法: 40 只雄性Wistar大鼠随机分为安静组、小强度运动组(50% O₂max)、中强度运动组(65% O₂max)、大强度运动组(80% O₂max),每组10只。经3天适应性训练后测试大鼠最大摄氧量。运动组大鼠每天训练1 h,每周5天,共12周。训练期间每隔2周测试一次最大摄氧量。经过12周训练,于最后一次运动后48 h宰杀所有大鼠,取比目鱼肌(右侧)。采用RT-qPCR法测定AMPK、PGC-1α基因表达,采用Western blot法测定AMPK、PGC-1α、磷酸化AMPK蛋白表达。结果: 65% O₂max组和80% O₂max组最大摄氧量提升较快,而50% O₂max组在干预后期才出现明显升高;12周运动干预后,50% O₂max组和80% O₂max组PGC-1α mRNA表达显著高于安静组和中强度组(P<0.01),安静组和80% O₂max组AMPK mRNA表达显著低于中强度组(P<0.05,P<0.01);运动组PGC-1α蛋白表达高于安静组,其中65% O₂max组相比安静组具有显著性差异(P<0.05);AMPK蛋白表达组间相比没有显著差异,但中强度运动组和大强度运动组磷酸化AMPK相比安静组有所增加,大强度运动组增加幅度更大,显著高于安静组和小强度运动组(P<0.05)。结论: 随着运动强度的增加,骨骼肌磷酸化AMPK表达增加;运动强度越大不代表PGC-1α蛋白表达会越高;中强度运动对AMPK/PGC-1α信号通路的影响可能更有益于心肺耐力的提升。

关键词: AMPK, PGC-1α, 运动强度, 心肺耐力

Abstract: Objective: To investigate the biological mechanism of exercise to improve cardiorespiratory fitness (CRF) through exercise based on 12 weeks of exercise intervention with different intensities on the skeletal muscle AMPK/PGC-1α pathway in rats. Methods: 40 male Wistar rats were randomly divided into four groups with 10 in each group, i.e. sedentary group, low intensity group (50%O₂max), moderate intensity group (65%O₂max), and high intensity group (80% O₂max). All rats were measured for the O₂max after three days adaptive training. After that, the rats in the exercise groups were trained for 12 weeks, 5 day a week, and 1hour per day. They were tested for O₂max every two weeks during the training period. All rats were sacrificed 48 hours after the last exercise and their soleus (right) was isolated. The AMPK and PGC-1αgene expression were detected by RT-qPCR while the AMPK,PGC-1α,and phosphorylated AMPK protein expression by Western Blot. Results: The O₂max in 65% O₂max group and 80% O₂max group increased rapidly, while the O₂max in 50%O₂max group increased significantly in the late stage of intervention. After 12 weeks of intervention, the PGC-1α mRNA expression in 50%O₂max group and 80%O₂max group were significantly higher than that in sedentary group and moderate intensity group (P<0.01), the AMPK mRNA expression in sedentary group and 80%O₂max group were significantly lower than that in 65%O₂max group (P<0.05, P<0.01). The PGC-1α protein expression in the exercise groups was higher than that in sedentary group, with only the PGC-1α protein expression in 65%O₂max group being significantly higher than that in sedentary group (P<0.05). There was no significant difference in AMPK protein expression among the groups, while the phosphorylated AMPK protein expression in moderate intensity group and high intensity group increased as compared with sedentary group. The increase in 80%O₂max group was greater, significantly higher than that in sedentary group and low intensity group (P<0.05). Conclusions: Higher exercise intensity does not mean that PGC-1α protein expression will increase correspondingly. The effect of moderate intensity exercise on the AMPK/PGC-1α signaling pathway may be more beneficial for CRF.

Key words: AMPK, PGC-1α, exercise intensity, CRF

中图分类号:

G804.2

许杰,黄巧婷,谢敏豪,严翊,林家仕. 不同强度运动对大鼠骨骼肌AMPK/PGC-1α信号通路的影响[J]. 成都体育学院学报, 2018, 44(4): 121-126.

XU Jie, HUANG Qiaoting, XIE Minhao, YAN Yi, LIN Jiashi. Effects of Different Intensity Exercise on AMPK/PGC-1α Signaling Pathway in Skeletal Muscle of Rats[J]. Journal of Chengdu Sport University, 2018, 44(4): 121-126.

[1]	顾正秋, 黎涌明. 课次主观疲劳度的信效度及方法学影响因素[J]. 成都体育学院学报, 2024, 50(1): 143-151.
[2]	王世强, 郭凯林, 吕万刚. 体力活动对中国老年人衰弱的影响——基于中国健康与养老追踪调查的实证分析[J]. 成都体育学院学报, 2023, 49(5): 114-122.
[3]	柯友枝, 张丹青, Grant Tomkinson, 刘阳. 儿童青少年有氧耐力变化趋势的分析及启示——基于1964—2017年的国内外研究[J]. 成都体育学院学报, 2023, 49(3): 77-84.
[4]	吴华, 张新定, 阮辉, Wichai Eungpinichpong. 国外心肺耐力/心肺适能与学前儿童健康相关性研究的系统综述[J]. 成都体育学院学报, 2021, 47(6): 130-137.
[5]	郭辉, 孙景权, 张一民. 六分钟上下楼梯试验与摄氧量的相关性研究[J]. 成都体育学院学报, 2016, 42(3): 14-18.

不同强度运动对大鼠骨骼肌AMPK/PGC-1α信号通路的影响

Effects of Different Intensity Exercise on AMPK/PGC-1α Signaling Pathway in Skeletal Muscle of Rats

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 5

编辑推荐

Metrics

本文评价