Abstract: The purpose of the paper is to explore the characteristics of knee joint muscle strength of outstanding female wushu athletes. Isokinetic muscle strength testing method is used to conduct a quantitative test on four outstanding female wushu athletes’ left and right knees for their slow 60°/s and 240°/s bending and stretching. The results show that the four subjects exhibit little change in their flexor group, but a relatively more decrease in their extensor group. Compared with 60°/ s test, the best powering angle of the knee joint appears earlier in the process of fast flexion and extension. The paper draws the conclusion that two of the subjects develop better in their knee joint strength with balanced flexion and extension muscle strength and special characteristics. Another subject shows a bigger different muscle group rate on the opposite side of the same name than 10%, so he needs to strengthen the anterior muscle group of right knee joint, and the last one needs to exercise the explosive power of his left knee joint flexor muscles.