补充L-肉碱结合运动对肥胖小鼠骨骼肌FAT/CD36及CPT-1的影响

成都体育学院学报 ›› 2014, Vol. 40 ›› Issue (8): 77-82.

补充L-肉碱结合运动对肥胖小鼠骨骼肌FAT/CD36及CPT-1的影响

张喆^1,2,3, 张蕴琨², 季浏^1,3

1.青少年健康评价与运动干预教育部重点实验室, 上海 200241;
2.南京体育学院, 江苏南京 210014;
3.华东师范大学体育与健康学院, 上海 200241

收稿日期:2014-03-08 出版日期:2014-08-20 发布日期:2014-09-01
作者简介:张喆(1986-), 女, 重庆人, 博士研究生, 硕士学位, 研究方向:运动对健康作用的细胞分子机制研究。
基金资助:
江苏省科技厅基础研究计划(自然科学基金)项目编号:BK2011863;青少年POWER工程协同创新中心项目,项目编号:44801400/012。

The effect of L-carnitine and exercise on obese mice FAT/CD36 and CPT-1 enzyme in skeletal muscle

ZHANG Zhe, et al

Key Laboratory of Adolescent Health Assessment and Exercise Intervention of the Ministry of Education, Shanghai, 200241

Received:2014-03-08 Online:2014-08-20 Published:2014-09-01

摘要/Abstract

摘要： 目的:研究补充L-肉碱结合运动对营养型肥胖小鼠骨骼肌FAT/CD36以及CPT-1的影响。方法:49只C57BL/6雄性小鼠,随机选取9只喂养普通饲料(C组),另外40只建立肥胖模型(H组)。建模成功后将小鼠随机分为肥胖安静组(H)、肥胖肉碱组(HL)、肥胖运动组(HE)、肥胖肉碱结合运动组(HLE)。HE、HLE组进行为期5周的游泳训练,5天/周,1小时/天。HL、HLE组小鼠每天训练前1小时灌胃L-肉碱。实验期间监测小鼠体重变化,实验结束后取腓肠肌检测相关指标。结果:①HE、 HLE组体重低于H组,且HLE组体重低于HL组;②HE、HL组和HLE组的FFA含量显著低于H组,而HE、HLE组的FFA含量也明显低于HL组;③HL、HE组和HLE组的CPT-1酶含量显著低于H组;④HE组的FAT/CD36表达量高于H组,HLE组则低于H组;⑤CPT-1酶含量和FAT/CD36表达量与体重均呈中度正相关。以上差异均具有统计学意义,P<0.05或P<0.01。结论:①与补充L-肉碱相比,运动和运动结合L-肉碱有更显著的控体重效果和促进脂肪酸氧化的作用。②单纯运动,补充L-肉碱以及运动结合L-肉碱均可降低骨骼肌内CPT-1酶的含量。③运动结合左旋肉碱可交互促进降低FAT/CD36的表达量。

关键词: L-肉碱, 运动, CPT-1酶, FAT/CD36

Abstract: Objective: To study the effects of L-carnitine combined with exercise on FAT/CD36 and CPT-1 of obese mice. Methods: Male C57BL / 6 mice were randomly assigned to group C (fed with normal diet) and group H (fed with high-fat diet). After group H was modeled successfully, they were randomly divided into group H (obesity quiet), group HL (obesity with L-carnitine), group HE (obesity with exercise), group HLE (obesity with L-carnitine combined exercise). Swimming training was adopted to them, 1 hour / day, 5 days / week, lasting for five weeks. Group HL, HLE were gavaged with L-carnitine an hour before training. During the experiment, changes of body weight of mice were monitored. After the experiment, the gastrocnemius muscles were prepared to determine the content of FFA, CPT-1 and FAT/CD36. Results: ① After 5 weeks of training, the body weight of group HE and HLE became lower than group H, and the weight of group HLE was also lower than group HL; ② the FFA of group HL, HE and HLE was significantly lower than in group H, and in group HE and HLE it was also significantly lower than group HL; ③ the contents of CPT-1 of group HL, HE and HLE were significantly lower than in group H; ④ the expression of FAT/CD36 of group HE was higher than in group H, HLE group was lower than group H; ⑤ the content of CPT-1 and FAT/CD36 showed a moderate positive correlation with body weight. These differences were statistically significant, P <0.05 or P <0.01. Conclusion: ① there is no apparent role of pure L-carnitine supplementation in controlling weight. However, there is significant effect of exercise and exercise combined with L-carnitine on controlling weight. ② the effect of exercise and exercise combined with L-carnitine in promoting metabolism and oxidation of fatty acid is more obvious than pure L-carnitine supplementation. ③ L-carnitine, exercise, and exercise combined with L-carnitine can reduce CPT-1 levels. ④ exercise combined with L-carnitine can interactively reduce the expression of FAT/CD36. The contents of CPT-1, FAT/CD36 are moderately correlated with body weight.

Key words: L-carnitine, exercise, CPT-1, FAT/CD36

中图分类号:

G804

张喆, 张蕴琨, 季浏. 补充L-肉碱结合运动对肥胖小鼠骨骼肌FAT/CD36及CPT-1的影响[J]. 成都体育学院学报, 2014, 40(8): 77-82.

ZHANG Zhe, et al. The effect of L-carnitine and exercise on obese mice FAT/CD36 and CPT-1 enzyme in skeletal muscle[J]. 成都体育学院学报, 2014, 40(8): 77-82.

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