抗阻训练与耐力训练对小鼠腓肠肌卫星细胞激活作用的影响

成都体育学院学报 ›› 2014, Vol. 40 ›› Issue (4): 55-59.

抗阻训练与耐力训练对小鼠腓肠肌卫星细胞激活作用的影响

陈彩珍^¹，²，卢健^¹，²

1.青少年健康评价与运动干预教育部重点实验室华东师范大学，上海200241；
2.华东师范大学体育与健康学院，上海200241

收稿日期:2014-05-16 修回日期:2014-05-16 出版日期:2014-04-20 发布日期:2014-05-16
作者简介:陈彩珍(1967-)，女，福建上杭人，教授，博士，研究方向运动训练的生物学适应。
基金资助:
上海市浦江人才计划资助项目(项目编号10PJC029)。

Effect of Resistance and Endurance Training on the Activation of Muscle Satellite Cells in the Gastrocnemius of Mice

CHEN Cai zhen^¹,², LU Jian^¹,²

1. Key Laboratory of Adolescent Health Assessment and Exercise Intervention, Ministry of Education,East China Normal University,Shanghai 200241;
2. College of Physical Education and Health, East China Normal University, Shanghai 200241

Received:2014-05-16 Revised:2014-05-16 Online:2014-04-20 Published:2014-05-16

摘要/Abstract

摘要： 通过观察骨骼肌肥大相关细胞因子mRNA的表达，探讨抗阻训练或耐力训练对骨骼肌卫星细胞增殖分化能力的作用及可能机制。选取21只雄性老年SAMP8小鼠，随机分为对照组(C)，耐力训练组(E，8周跑台训练)，抗阻训练组(R，8周尾部负重爬梯训练)。经过8周的训练后，检测小鼠右侧腓肠肌横截面积及IGF-1、MGF、MyoD及 myogenin等mRNA的表达。结果发现耐力训练和抗阻训练大鼠腓肠肌IGF-1、MGF、MyoD及 myogenin等mRNA的表达均显著高于对照组;两训练组间比较，抗阻训练组MGFmRNA显著高于耐力训练组(P<0.01)，其它3个基因的mRNA有上调趋势，但无显著性差异;抗阻训练组的腓肠肌纤维横截面积显著高于对照组和耐力训练组。抗阻训练在维持肌质量方面效果优于耐力训练，推测这两种不同的运动方式对腓肠肌质量影响之差异可能与MGF介导的骨骼肌卫星细胞的激活有关。

关键词: 骨骼肌肥大, IGF-1, 生肌调节因子, 耐力训练, 抗阻训练

Abstract: Objective To observe the effect of endurance training or resistance training on the expression of IGF-1、MGF、MyoD and myogenin mRNA in gastrocnemius muscle. and to explore its role in the activation of muscle satellite cells. Methods 21 SAMP8 senile male mice were randomly distributed into three groups sedentary (CON, n=7) , endurance training group (n=7), resistance training groups (n=7). After 8wk training, gastrocnemius muscles were excised and muscle-wet weight and body mass (BM) are weighed and analyzed by real-time PCR. Results The ratio of muscle wet weight and BM (RM) of resistance training group showed significant differences when compared with that of the endurance group or control group ,8wk endurance training or resistance training resulted in an increase in mRNA levels of IGF-1、MGF、MyoD and myogenin, whereas two training groups have no significant difference except IGF-1mRNA which were significantly higher in resistance training group than those in endurance group. Conclusion Resistance training is better than endurance training in maintaining muscle quality effect. Two different exercise ways might be through the MGF which represent potential mediators implicated in the regulation of hypertrophy during exercise to activate the muscle satellite cells.

Key words: hypertrophy, IGF-1, MRFs, endurance training, resistance training

中图分类号:

G804.2

陈彩珍^，，卢健^，. 抗阻训练与耐力训练对小鼠腓肠肌卫星细胞激活作用的影响[J]. 成都体育学院学报, 2014, 40(4): 55-59.

CHEN Cai zhen^,, LU Jian^,. Effect of Resistance and Endurance Training on the Activation of Muscle Satellite Cells in the Gastrocnemius of Mice[J]. , 2014, 40(4): 55-59.

[1]	王漫卓, 苏文, 刘艳环, 马国栋. 耐力训练诱导的肥胖大鼠Irisin抑制脂肪组织FTO表达[J]. 成都体育学院学报, 2017, 43(1): 113-119.
[2]	胥靓，，伊木清，楼望，李伟军，周丽丽，许葆华，付劲德，高红. 耐力训练和适量限食轻度上调大鼠骨骼肌ER分子伴侣表达并改善糖代谢的研究[J]. 成都体育学院学报, 2013, 39(5): 84-90.
[3]	陈金鳌，张霈，杨帆，陆阿明. 青年和老年男女抗阻训练后的骨密度变化特征[J]. 成都体育学院学报, 2013, 39(2): 83-88.