Abstract: Objective: To study the changes of oxygen uptake, HR,energy expenditure, RPE, Tc (core temperature, Tc), SF (stride frequency, SF) and other related physiological parameters in real-time during uphill brisk walking and uphill jogging with the same speed. Method: 10 healthy males (20-25 yrs old) were randomly selected as subjects to fulfill both uphill brisk walking and jogging (treadmill speed: 6.4km/h, gradient: 10%, time duration: 40min) in the experiment. MOXUS gas analysis system and core temperature detection system were used to collect physiological parameters in real time. Result: (1) After 20min, the percentage of maximum oxygen uptake (%VO₂max) of uphill jogging group (UJG) was higher than that of uphill brisk walking group (UBWG) (P<0.05). (2) After 25min, the HR of UJG was higher than that of UBWG (P<0.05). (3) After 20min, the Tc of uphill jogging group was higher than uphill brisk walking group (P<0.05), at 25min (P<0.01). (4) In UJG, %VO₂max at both 35min and 40min were higher than that at 10min (P<0.05); Otherwise there was no such changes in UBWG (P>0.05). Conclusion: (1) Cardiopulmonary reaction and energy expenditure of UJG are more intense than those of UBWG. These differences could result from the higher stride frequency in UBWG . (2) The increase of Tc of UJG is higher than that of UBWG. The difference could result from higher levels of exercise intensity and energy expenditure of UJG.(3)During the 40min UJG and UBWG exercise, carbohydrates consumption was the same, but the fat consumption was higher in UJG.